Table 3 The expression of PV and miR-7 in infected A549 cell co-transfected with siRNA-100 plus siRNA-NC, or co-transfected with siRNA-100 plus siRNA-ago and siRNA-drosha, was determined by realtime PCR.

From: Host-virus interaction: the antiviral defense function of small interfering RNAs can be enhanced by host microRNA-7 in vitro

post-transfection hours:

24 h

48 h

72 h

PV (∆CT values)

siRNA-100 + siRNA-NC

0.23 ± 0.06

0.10 ± 0.06

−2.16 ± 0.12

 

siRNA-100 + siRNA-ago + siRNA-drosha

−3.19 ± 0.14**

−3.07 ± 0.18**

−3.89 ± 0.21*

miR-7 levels (molecules per cell)

siRNA-100 + siRNA-NC

44.07 ± 4.86

17.80 ± 0.84

0.32 ± 0.07

 

siRNA-100 + siRNA-ago + siRNA-drosha

5.69 ± 0.27**

5.01 ± 0.25**

0.13 ± 0.03**

  1. **p < 0.01, compared with siRNA-100 + siRNA-NC;
  2. *p < 0.05,compared with siRNA-100 + siRNA-NC.
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