Figure 3 | Scientific Reports

Figure 3

From: Common Oncogene Mutations and Novel SND1-BRAF Transcript Fusion in Lung Adenocarcinoma from Never Smokers

Figure 3

Functional Analyses of SND1-BRAF.

(A). Western blots of lysates from H1299 cells transfected with pCMV6-AC-GFP vector, pCMV6-AC-GFP-BRAFwt, pCMV6-AC-GFP-BRAFV600E, or pCMV6-AC-GFP-SND1-BRAF were probed with antibodies to C-terminus of BRAF, phospho-MEK1/2 (pMEK), total MEK (MEK), phospho-ERK1/2(pERK), or total ERK (ERK). (B). Proliferation assay of H1299 cells transfected as in A. Error bars represent the average ratio from triplicates. (C). Spheroid formation assay of H1299 cell transfected with indicated constructs. Error bars represent the standard error of the mean (SEM). Statistical significances are as indicated.

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