Figure 1 | Scientific Reports

Figure 1

From: DNA immunoprecipitation semiconductor sequencing (DIP-SC-seq) as a rapid method to generate genome wide epigenetic signatures

Figure 1

A flow-chart for the DNA immunoprecipitation semiconductor sequencing (DIP-SC-seq) protocol. Major steps in the DIP-SC-seq protocol are shown which can be broken into four stages. Step i is to enrich the DNA fragments of interest through antibody based pull downs. Step ii is low level whole genome amplification to generate double stranded DNA. Step iii is the preparation of sequencing libraries prior to sequencing on the semiconductor itself. And finally step iv is bioinformatic processing and normalisation. Overall it is possible to complete the entire protocol in less than 4 days.

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