Figure 4 | Scientific Reports

Figure 4

From: DNA immunoprecipitation semiconductor sequencing (DIP-SC-seq) as a rapid method to generate genome wide epigenetic signatures

Figure 4

HmeDIP-SC-seq derived 5hmC patterns closely resemble those derived through sequencing on the Illumina Hiseq platform. (a) The average 5hmC patterns across genic portions of the genome are plotted for replicate hmeDIP-SC-seq runs (red and pink) alongside those for hmeDIP-Hiseq (green) and affinity-Hiseq (blue). The average number of reads in a 200 bp window are then calculated across each gene before averaging across 22,093 genes loci. Genic loci are extended 25% upstream and downstream of each region to analyse the surrounding environment. (b) The genomic distributions of 5hmC enriched windows (“peaks”. see supplementary information) in the hmeDIP-SC-seq, hmeDIP-Hiseq and published affinity Hi-seq datasets were compared by mapping to one of the five indicated regions in the genome. Percentage of total peaks mapping to each locus are shown.

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