Figure 5 | Scientific Reports

Figure 5

From: DNA immunoprecipitation semiconductor sequencing (DIP-SC-seq) as a rapid method to generate genome wide epigenetic signatures

Figure 5

Examples of the utility of DIP-SC-seq. (a) 5hmC patterns generated by hmeDIP-SC-seq reflect toxicological insult over the Cyp2b10 locus. Genic 5hmC patterns are elevated following 91 days phenobarbital (PB) exposure (pink) compared to the control liver 5hmC (blue). This reflects a strong elevation in gene expression from this locus (data not shown). Box on the left highlights the promoter region of the Cyp2b10 gene and compares hmeDIP-SC-seq patterns to published 5hmC promoter microarrays. Regions which gain 5hmC upon drug expose are highlighted pink, regions which lose 5hmC are highlighted blue. (b) 5hmC patterns generated by hmeDIP-SC-seq can be used to track epigenetic changes which occur during reprogramming in cultured cells. Analysis of the 5hmC patterns over several candidate loci reveal IPSC specific loci (i), MEF specific loci (ii) as well as those which share a common 5hmC pattern (iii).

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