Figure 3
Sirt7 knockdown inhibits gastric cancer growth in vitro and in vivo.
(A) Representative western blot showing Sirt7 knockdown in MGC803 cells. (B-C) Sirt7 knockdown represses MGC803 cells proliferation. (B) Representative image by phasecontrast microscopy. Bar = 30 µm. (C) MTT analysis of cellular proliferation ability at the indicated time points. * p < 0.05 and ** p < 0.01 vs. sh-Ctrl. (D-E) Sirt7 knockdown inhibits colony formation of MGC803 cells. (D) Representative image showing cellular colonies of MGC803 cells with/without Sirt7 knockdown. (E) Quantitative results of colony numbers of different groups. (F-I) Sirt7 knockdown inhibits MGC803 cancer cells growth in vivo. (F) Representative image of subcutaneous tumors isolated from nude mice. (G) Quantitative analysis of tumor weights. n = 10. (H) Representative image of in vivo bioluminescence imaging indicating tumor size. (I) Quantitative analysis of total photon flux values. n = 10. MGC803 cells expressing luciferase were used in this experiment.
