Figure 5
Sirt7 knockdown up-regulates miR-34a expression.
(A) Sirt7 knockdown up-regulates the expression of miR-34a. (B) miR-34a is down-regulated in human gastric cancer tissues compared with adjacent gastric mucosa (AGM; n = 46 in each group). (C) Regression analysis showing the level of miR-34a is negatively correlated with the mRNA and protein levels of Sirt7 in human gastric cancer tissues (n = 46). The protein score was obtained from the IHC results, which indicates the percentage of Sirt7-positive area. (D) ChIP-qPCR data showing Sirt7 binds to the promoter region of miR-34a. ** p < 0.01 vs. IgG-ChIP; ## p < 0.01 vs. sh-Ctrl. (E) ChIP-qPCR data showing Sirt7 knockdown enhances H3K18 acetylation level at the promoter region of miR-34a. ** p < 0.01 vs. IgG-ChIP; ## p < 0.01 vs. sh-Ctrl. (F) Sirt7 and H3K18ac levels at miR-34a promoters in AGM and gastric cancer tissues. (G) Luciferase assay showing Sirt7 knockdown up-regulates the transcriptional activity of miR-34a.
