Figure 1
From: A versatile clearing agent for multi-modal brain imaging
TDE characterization.
(a) Transmission images of 1 mm thick hemi-brain slices of Thy1-GFP-M mouse in PBS and after clearing with various solutions. (b) Light transmittance curves of hemi-brain slices shown in a (mean ± s.e.m., n = 4). (c) Normalized linear deformation during optical clearing (mean ± s.d., n = 4). (d) Contrast decay as function of depth in uncleared and cleared samples (mean ± s.d., n = 10). (e) Half-time fluorescence decay (mean ± s.d., n = 4); clearing did not increase photobleaching compared to PBS. (f) Fluorescence intensity over time (mean ± s.d., n = 10); no quenching effect was observed after incubation of the sample in 47% TDE/PBS for up to two months. (g) Two-photon fluorescence imaging of 2 mm FVB mouse brain slices stained with DAPI in PBS and in 47% TDE/PBS. Reconstruction along z axis, depth 1 mm; scale bar = 100 µm. (h) TEM images of Thy1-GFP-M mouse brain slices previously incubated in PBS and in 47% TDE/PBS for 4 days. Triangles indicate mitochondria (red), axons (green) and nuclei (blue). Scale bar = 2 µm (upper panels) and 200 nm (lower panels).
