Table 1 Comparison of recently published clearing methods. Main characteristics of different clearing methods reported in literature. We considered adult mouse brain as typical sample. This table gives an overview of available techniques and their limitations and advantages.
From: A versatile clearing agent for multi-modal brain imaging
Method | Clearing composition | Refractive index | Linear deformation | Protein fluorescence quencing | Sample characteristic | High viscosity | Cost* | Clearing time | Immunostaining compatibility | Clearing capability | References |
Et + BABB | Ethanol Benzyl alcohol Benzyl Benzoate | 1.54 | Shrinkage | Yes | Stiff | No | $ | 2 days | No | Good | 7 |
THF + DBE | Thetrahydrofuran Dibenzyl ether | 1.56 | Shrinkage | Yes | Stiff | No | $ | 2 days | No | Good | 8 |
iDISCO | Thetrahydrofuran Dibenzyl ether | 1.56 | Shrinkage | Yes | Stiff | No | $$ | 2 days | Yes | Good | 9 |
Glycerol | Glycerol solution | 1.44 | No | No | / | Yes | $ | 2 days | No | Moderate | / |
Sca/e | Urea Glycerol Triton X-100 | 1.38 | Expansion | No | Fragile | No | $ | Months | No | Moderate | 10 |
SeeDB | Fructose saturated α thioglycerol | 1.49 | No | No | / | Yes | $ | 2 weeks | No | Moderate | 11 |
ClearT | Formamide Polyethylene glycol | 1.45 | No | No | / | No | $ | 1 day | No | Moderate | 12 |
CUBIC | Urea Aminoalcohols | 1.47 | Transient swelling | No | / | No | $$ | 2 weeks | Yes | Good | 13 |
CLARITY | FocusClear | 1.45 | Transient swelling | No | Spongy | No | $$$ | 10 days | Yes | Good | 14 |
CLARITY2 | PBST | 1.33 | / | No | Spongy | No | $$ | 12 days | Yes | Good | 21 |
TDE | 2,2' Thiodioethanol | Tunable | No | No | / | No | $ | 2 hours | No | Moderate | / |
CARITY + TDE | 2,2' Thiodioethanol | Tunable | Transient swelling | No | Spongy | No | $$ | 10 days | Yes | Good | / |
