Figure 3

Arrested endochondral ossification of Mt1:
(a) COLII expression during the development of Mt1 indicates the distribution of immature chondrocytes; (b) IHH expression during the development of Mt1 indicates the distribution of pre-hypertrophic chondrocytes; (c) Paraffin sections of Mt1 at HH34 and HH35 show the appearance of hypertrophic cells in the proximal side at HH35; (d) COLX expression during the development of Mt1 indicates the distribution of hypertrophic chondrocytes; (e) Calcein staining during the development of Mt1 showing the beginning of ossification; (f) Dorsal view of COLII (purple) and IHH (green) expression during the development of quail left metatarsi.