Figure 2
From: SIRT7 inactivation reverses metastatic phenotypes in epithelial and mesenchymal tumors
SIRT7 promotes cancer cell migration and invasiveness.
(A), Wound healing assay showing reduced migration of SIRT7-depleted (shSIRT7) PC3 cells. (B), Transwell assay showing reduced invasion of SIRT7 depleted PC3 cells. (C), 3D Matrigel assay showing reduced invasive growth of SIRT7 depleted cells through surrounding matrix. Images taken 3 days after plating of cells. (D), Phalloidin staining of F-actin revealed a more collapsed actin cytoskeleton in SIRT7-depleted PC3 cells compared to controls. (E), Impaired cell migration and wound healing in SIRT7- depleted HT1080 cells. (F), Impaired invasion of SIRT-depleted HT1080 cells in Transwell assay. (G), Reduced invasion of HT1080 cells overexpressing the catalytically inactive (S7-HY) SIRT7 protein in the Transwell assay.
