Figure 5
From: SIRT7 inactivation reverses metastatic phenotypes in epithelial and mesenchymal tumors
SIRT7 physically interacts with SIRT1 and is required for SIRT1-mediated repression of E-cadherin.
(A), Western analysis showing co-immunoprecipitation (IP) of Flag-tagged SIRT7 and endogenous SIRT1. (B), Western analysis showing co-IP of Flag-tagged SIRT1 and endogenous SIRT7. (C), Western analysis showing co-IP of endogenous SIRT1 and SIRT7 proteins. (D), q-PCR analysis of the E-cadherin expression following overexpression of wild-type (S1WT) or catalytically inactive (S1HY) SIRT1 proteins in Control or SIRT7-depleted (shSIRT7) PC3 cells. (E), Western blot showing E-cadherin protein levels from cells used in D. (F), Western blot analysis showing co-immunoprecipitation of SIRT7 by the SIRT1 WT and HY mutant proteins. Western Blots were carried under same experimental conditions. Uncropped images are available in supplementary figure 1c.
