Figure 1
From: TIGAR regulates DNA damage and repair through pentosephosphate pathway and Cdk5-ATM pathway
TIGAR knockdown increased DNA damage.
Knockdown of TIGAR in HepG2 cells was achieved with transient transfection of TIGAR siRNA. Forty-eight h after transfection, HepG2 cells were then treated with 200 mM CoCl2 or 2.5 μg/ml epirubicin for 10 h and 12 h, respectively. (a) CoCl2-induced DNA damage in TIGAR knockdown HepG2 cells. Left: representative images of Comet assay. Right: quantification of Comet tail DNA% and tail length. (b) Epirubicin-induced DNA damage in TIGAR knockdown HepG2 cells. Left: representative images of Comet assay. Right: quantification of Comet tail DNA% and tail length. (c) Distribution of γ-H2AX in HepG2 cells treated with 200 nM CoCl2 or 2.5 μg/ml epirubicin after TIGAR knockdown. HepG2 cells were treated as described above and were analyzed with a confocal microscopy. γ-H2AX was stained red and the nucleus was stained blue. Scale bar = 25 μm. Values are means ± SD from 3 independent experiments. *** p < 0.001; ns, p > 0.05 versus control group; ### p < 0.001 versus corresponding groups.
