Figure 2
From: TIGAR regulates DNA damage and repair through pentosephosphate pathway and Cdk5-ATM pathway
TIGAR knockdown increased DNA damage through increases in ROS.
HepG2 cells or TIGAR knockdown HepG2 cells were all treated with CoCl2 (200 nM) for 10 h or epirubicin (2.5 μg/ml) for 12 h and NAC, NADPH or ribose were added 2 h before the treatment of CoCl2 or epirubicin. DNA damage was detected with the Comet assay. (a) G6PD expression detected with Western blot analysis after TIGAR knockdown in HepG2 cells. GAPDH was used as a loading control. Quantitative analysis was performed with Image J. (b) DNA damage in TIGAR knockdown HepG2 cells treated with CoCl2 combined with or without treatment of NAC or NADPH. Left panel: representative images of Comet assay. Right panel: quantification of Comet tail DNA% and tail length. (c) DNA damage in TIGAR knockdown HepG2 cells treated with epirubicin combined with or without treatment of NAC. Left panel: representative images of Comet assay. Right panel: quantification of Comet tail DNA% and tail length. Values are means ± SD from 3 independent experiments. *** p < 0.001, ns p > 0.05 versus corresponding Groups.
