Figure 3
From: TIGAR regulates DNA damage and repair through pentosephosphate pathway and Cdk5-ATM pathway
TIGAR knockdown increased DNA damage by reducing PPP.
HepG2 cells or TIGAR knockdown HepG2 cells were treated with CoCl2 (200 nM) for 10 h or epirubicin (2.5 μg/ml) for 12 h and NADPH or ribose was added 2 h before the treatment of CoCl2 or epirubicin. (a) DNA damage in TIGAR knockdown HepG2 cells or control cells treated with epirubicin combined with NADPH. Upper panel, representative images of Comet assay. Lower panel, quantification of Comet tail DNA% and tail length. (b) DNA damage in TIGAR knockdown HepG2 cells treated with epirubicin combined with or without ribose. Upper panel, representative images of Comet assay. Lower panel, quantification of Comet tail DNA% and tail length. (c) DNA damage in TIGAR knockdown HepG2 cells or control cells treated with CoCl2 combined with NADPH, ribose alone or both. Upper panel, representative images Comet assay. Lower panel, quantification of comet tail DNA% and tail length. (d) DNA damage in TIGAR knockdown HepG2 cells or control cells treated with epirubicin combined with NADPH and ribose. Upper panel, representative images of Comet assay. Lower panel, quantification of Comet tail DNA% and tail length . Values are means ± SD from 3 independent experiments. *** p < 0.001, ns p > 0.05 versus control group.
