Figure 4
From: TIGAR regulates DNA damage and repair through pentosephosphate pathway and Cdk5-ATM pathway
The nuclear translocation of TIGAR under genome stress or hypoxia condition.
(a) The nucleus translocation of TIGAR after treatment of HepG2 cells with 200 mM CoCl2 for 8 h. Fluorescence intensity of TIGAR in the nucleus was detected with a confocal microscopy. TIGAR was stained red and the nucleus was stained blue. Scale bar = 25 μm. (b) The nuclear translocation of TIGAR after treatment of 2.5 μg/ml epirubicin for 12 h. Fluorescence intensity of TIGAR in the nucleus was detected with a confocal microscopy. TIGAR was stained red and the nucleus was stained blue. Scale bar = 25 μm. (c) The nuclear TIGAR protein after treatment with CoCl2. The nuclear proteins were extracted and TIGAR protein level in the nucleus and cytosol was detected with Western blot analysis. Histon-H3 was used as a loading control for the nuclear protein and GAPDH was used as a loading control for cytosolic proteins. Quantitative analysis was performed with Image J. (d) The nuclear TIGAR protein after treatment with epirubicin. The nuclear proteins were extracted and TIGAR protein level in the nucleus and cytosl was detected with Western blot analysis. Histon-H3 was used as a loading control for the nuclear proteins and GAPDH was used as a loading control for the cytosolic proteins. Quantitative analysis was performed with Image J. Values are means ± SD from 3 independent experiments. * p < 0.05 versus control group.
