Figure 15
Representative TEM micrographs and A11 dot blot images showing aggregate morphology and toxicity of A53T mutant α-synuclein in the absence or presence compounds 1, 3, 6 and 15.
(a) TEM images of A53T mutant α-synuclein (210 µM) aggregated for 30 days (A) alone or in the presence of equimolar concentrations of (B) compound 1 (C) compound 3 (D) compound 6 (E) compound 15. Scale bar is 200 nm. (b) A11 dot blots showing effect of compounds 1, 3, 6 and 15 on A53T mutant α-synuclein A11 epitope formation. A53T mutant α-synuclein samples (210 µM) were aggregated alone or co-incubated with equimolar concentration of compounds 1, 3, 6 and 15 for 30 days or 60 days. Compounds were added on day 0 and day 30 respectively.
