Figure 1

Establishment of dendritic cells co-expressing the hNIS and effluc genes.
(a) Flow cytometric analysis of parental DC2.4 and DC/NF cells using an APC-Cy7-conjugated CD90.1 antibody. (b) RT-PCR analysis to determine the expression of the hNIS and effluc genes in parental DC2.4 and DC/NF cells. Gapdh was used as an internal control gene. (c) In vitro radioiodine uptake in DC2.4 and DC2.4/NF cells. (d) In vitro luciferase assay in parental DC2.4 and DC2.4/NF cells. Data are expressed as the meanā±āstandard deviation (SD) of 3 independent experiments.