Figure 3 | Scientific Reports

Figure 3

From: Tracking of dendritic cell migration into lymph nodes using molecular imaging with sodium iodide symporter and enhanced firefly luciferase genes

Figure 3

Effect of transduction of the NIS gene on DC function.

(a) Cell proliferation rates in parental DC2.4 and DC/NF cells. There was no significant difference between the two cell lines. (b) Phenotypic analysis of DC2.4 and DC2.4/NF cells. Both DC2.4 and DC2.4/NF cells were stained with PE-conjugated CD54, CD86, H-2Kb (MHC Class I) and I-A/I-E (MHC class II) and APC-conjugated CD205 (DEC-205), respectively. Red histograms represent the isotype control. (c) In vivo BLI of tumor progression using the Rluc gene. (d) Quantification of the BLI signal emitted from the tumor lesion on days 1 and 14 post-tumor challenge. Marked inhibition of tumor formation is observed with immunization of E7-transfected DC/NF cells. Data are expressed as the mean ± standard deviation (SD) of 3 independent experiments.

Back to article page