Figure 11

(A) The cartilage and bone portions of the reparative tissues were segmented using the thresholding function and their respective 3D volumes were computed from the segmented region of interest (ROI); (B) Semi-quantitative analysis the relative amount of bone and cartilage tissues formation in the critical size cartilage defect model based on Micro-CT images; (C) Histological scoring for reparative tissues. The reparative tissues were evaluated for cell morphology, matrix-staining, surface regularity, subchondral bone reconstruction, filling of defect and integration of donor with host adjacent cartilage. (a, b and c indicate blank group, scaffolds group and scaffolds + cells group. *p < 0.05, ** p < 0.01).