Figure 2

AEA suppresses MAPK phosphorylation in either Tat B or Tat C transfected Müller glia. Tat B transfected (a) Müller glia shows peak phosphorylation of ERK1/2 and JNK, at 2 hours, but at 1 hour in Tat C (b) cells. Peak phosphorylation of p38 occurred at 1 hour in Tat B cells but at 2 hours in Tat C cells (a and b). In AEA exposed cells, peak phosphorylation occurs earlier on transfection with Tat B than in Tat C. Levels of phosphorylation were suppressed in AEA treated cells relative to Tat alone, for both variants. Normalized levels of phosphorylated ERK1/2, JNK, p38 are shown as mean ± S.E (n = 3). Representative blots shown. Representative blots were cropped. Full-length blots are presented in Supplementary Fig. S6. *p < 0.05, **p < 0.01, ***p < 0.001 vs. Control.