Figure 3
From: Differential immune mechanism to HIV-1 Tat variants and its regulation by AEA
The STAT-SOCS feedback loop is regulated in a clade specific manner by AEA in activated Müller glia. Tat B and Tat C transfection affect STAT and SOCS in a differential manner. STAT levels are low when SOCS is high for both Tat variants, 8 hours onward (a, b). SOC-3 levels show negligible variation in either Tat variant, alone (c). AEA treatment lead to suppression of STAT levels for both variants (d). Exposure to AEA in Tat B cells lead to rise in SOCS-1 level, 30 min onward (e). Tat C + AEA treatment showed negligible effect on levels of SOCS-1 but increase in SOCS-3, 30 min onward (f). Normalized levels of phosphorylated STAT-1α, SOCS-1 and SOCS-3 are shown as mean ± S.E (n = 3). Representative blots shown. Representative blots were cropped. Full-length blots are presented in Supplementary Fig. S6. *p < 0.05, **p < 0.01,***p < 0.001 time points vs. 0 min.
