Figure 5

AEA acts in Tat B transfected cells through MKP-1 but in Tat C transfected cells through MEK. Tat B transfection induced sustained and elevated phosphorylation of MEK from 6 hours onward (a). Tat C induced phosphorylation of MEK was lower at all time points and fell after reaching peak at 6 hours (b). Effect of AEA in Tat C cells on the phosphorylation of MEK was more pronounced than on Tat B cells (c). Both Tat variants induced MKP-1 level 2 hours onward (e). Increase in MKP-2 occurs only with Tat B but not Tat C (f). AEA exposure affects MKP-1levels in a sustained manner in Tat B cells, while in Tat C + AEA cells, MKP levels rise early and fall thereafter (g). AEA has pronounced effect on Tat B cells but not Tat C cells (h). Normalized levels of phosphorylated MEK-1/2 with MEK-1/2 and MKP-1 as well as MKP-2 with β-actin are shown as mean ± S.E (n = 3). Representative blots shown. Representative blots were cropped. Full-length blots are presented in Supplementary Fig. S6. *p < 0.05, **p < 0.01, ***p < 0.001 vs 0 min.