Figure 7

Tat B and Tat C differentially affect NF-kB signalling. Nuclear NF-κB significantly increased on transfection with Tat B or Tat C. Addition of AEA significantly reduced the expression of nuclear NF-κB levels. Addition of CB1/2 blockers reversed the effect of AEA on Tat B/C transfected cells (a). Phosphorylated IκB increased enormously on transfection of Tat B. On transfection with Tat C, pIkB expression showed mild increase. AEA on Tat B cells reduced the expression of pIκB. Nominal down regulation of pIκB was seen in Tat C cells with AEA. Incubation with CB blockers partially reverses AEA effect (b). Inhibition with MAPK blockers significantly reduced Tat B + AEA effect on pIκB. In Tat C + AEA cells, significant reversal of AEA effects on pIκB was observed only on blocking with ERK inhibitor FR180204 (c). IRAK1BP1 levels increased significantly on Tat C transfection but not Tat B. Incubation with AEA on Tat B/C cells significantly increased IRAK1BP1 levels. Reversal by CB blockers of AEA effects was more pronounced for Tat B + AEA (d). TAB-2 increased with Tat B/C transfection. Addition of AEA reduces TAB2 significantly. Addition of CB blockers partially reverses effect on Tat B cells but not Tat C cells (e). TTP level increased significantly on transfection of Tat C. Addition of AEA increased TTP significantly only in Tat B cells. (f). Addition of CB blockers reverse AEA effect on Tat B cells but not in Tat C cells. Representative blots shown. Representative blots were cropped. Full-length blots are presented in Supplementary Fig. S6. Results shown are the mean ± S.E (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001 vs. Control. $$$p < 0.001 vs Tat B. &p < 0.05, &&p < 0.01, &&&p < 0.001 vs Tat C. ##p < 0.01, ###p < 0.001 vs. Tat B + AEA. + p < 0.5, ++ p < 0.01, +++ < 0.001 vs. Tat C+AEA.