Figure 8

Tat-induced adhesion of PBMC to Müller cells is inhibited by AEA. Tat B or Tat C induced PBMC adhesion to Müller cell line MIO-M1. Antagonists U0126, Ro- 318220, AM-251 and AM-630 alleviated PBMC adhesion, but not to the extent of AEA. CB blockers reversed the effect of AEA and increased PBMC adhesion. MKP-1 inhibition has a striking effect on Tat B + AEA cells but nominally affects Tat C + AEA cells compared to either Tat B + AEA or Tat C + AEA. MEK-1 inhibition affects adhesion to a significant extent only in Tat C + AEA cells, but not Tat B + AEA. MIO-M1 cells were transfected with Tat B or Tat C and then treated with AEA. MEK-1 or MKP-1 blocker (U0126 and Ro-318220, respectively), CB blockers (AM-251 or AM-630) were used simultaneously for 24 hours, wherever used (b). Adherent cells were expressed as arbitrary values. Transfection with empty plasmid or AEA and blockers to CB receptors, MEK-1 and MKP-1 alone do not induce PBMC adhesion to MIO-M1 (a). Results are expressed as mean ± S.E (n = 3). ***p < 0.001 vs Tat B, &&p < 0.01, &&&p < 0.001 vs Tat C, ###p < 0.001 vs Tat B + AEA, $p < 0.05, $$$p < 0.001 vs Tat C + AEA.