Figure 3
From: Multiple myeloma acquires resistance to EGFR inhibitor via induction of pentose phosphate pathway
EGFR inhibition enhances glucose uptake and suppresses oxygen consumption.
A) glucose uptake and lactate secretion measured by YSI in NRAS Mut and WT myeloma cells treated with Gefitinib (Gef, 5 μM) or control for 24 h or B) infected with shRNAs against the (Middle: shEGFR#1; Right: shEGFR#2). Bars, average of 3 independent samples ± SD, **P < 0.01; C) intact cellular respiration measured using the Seahorse Bioscience XF24 analyzer, under basal conditions or in the presence of FCCP in triple WT myeloma cells treated with Gefitinib (5 μM) for 24 h. Levels of oxygen consumption were normalized to cell number. Bars, average of 3 independent experiments ± SD. *P < 0.05.
