Figure 3 | Scientific Reports

Figure 3

From: The Tomato yellow leaf curl virus V2 protein forms aggregates depending on the cytoskeleton integrity and binds viral genomic DNA

Figure 3

V2 forms complexes with TYLCV genomic DNA. Detection of TYLCV DNA-V2 complexes in vitro (a) and in planta (b). a: PCR detection of viral DNA following immuno-capture with V2 antibody of mixtures of V2 and infected plant DNA; V2/DNA: V2 incubated with untreated DNA, V2/DNA + S1: V2 incubated with DNA treated with S1 nuclease, V2/-DNA: V2 incubated without DNA. M - 100 bp DNA marker. b: Southern blot analysis of viral DNA-V2 complexes from infected tomato plants; DNA: DNA from infected plants, V2/DNA: DNA extracted from immunoprecipitated V2 complexes, V2/DNA + S1: DNA extracted from immunoprecipitated V2 complexes and treated with S1 nuclease.

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