Table 3 Parameters for selected model fits (with selected fits depicted in Fig. 3) for binding of AT1R-H8 to lipid bilayers.

From: Helix 8 of the angiotensin- II type 1A receptor interacts with phosphatidylinositol phosphates and modulates membrane insertion

Lipid, Model

m * p

ka 1

k d1 x 10 −3

k a2 x 10 −3

k d2 x 10 −3

k a3 x 10 −3

k d3 x 10 −3

n 1 x 10 −3

n 2 x 10 −3

n 3 x 10 −3

e L

T m

x 10 −3

fit

DMPC 2-state

0.575 (0.559–0.598)

16905 (13473–21741)

24.0 (15.3–37.9)

5.56 (5.14–6.01)

0.20 (0–0.76)

  

−6.75 (−7.16–6.38)

−7.38 (−7.67–7.12)

     

0.389

DMPC (Fig. 3a)3-state + bilayer expansion

0.656 (0.629–0.683)

14682 (12683–17317)

56.6 (48.3–71.8)

10.43 (8.13–11.91)

0.68 (0.33–0.98)

3.29 (1.91–5.37)

0 (0–6.6)

−7.26 (−7.66–6.83)

−6.34 (−6.81–5.82)

−5.83 (−7.44–4.20)

0.34 (0.24–8.65)

   

0.177

DMPC/DMPS (Fig. 3b) 2-state

0.957 (0.932–0.990)

3416 (3209–3638)

7.0 (6.3–9.6)

5.1 (4.7–5.7)

0 (0–0.38)

  

−6.05 (−6.24–5.85)

−7.30 (−7.45–7.15)

     

0.105

DMPC/DMPS 3-state + bilayer expansion

1.152 (1.091–1.207)

3063 (2841–3305)

20.6 (17.9–24.5)

15.2 (13.7–16.5)

0 (0–15.1)

5.4 (4.8–6.8)

0 (0–2.4)

−6.27 (−6.52–5.94)

−6.32 (−6.51–6.07)

−4.98 (−5.23 –4.85)

0.35 (0.33–0.40)

   

0.056

DMPC/DMPS/PI(4)P (Fig. 3c) 3-state + expansion + lag

5.846 (5.220–6.349)

572 (504–600)

0 (0–2.6)

19.5 (17.8–21.4)

1.3 (0–5.4)

13.8 (13.1–14.4)

10.2 (9.3–11.2)

8.17 (6.36–9.71)

−18.54 (–19.71–17.18)

10.11 (8.69–11.71)

0.96 (0.88–1.01)

  

106.7 (94.9–119.2)

0.319

DMPC/DMPS/PI(4)P 3-state + expansion + threshold

3.902 (3.633–4.330)

1847 (1804–1927)

103.7 (8.2–152.7)

500 (273–500)*

33.7 (20.4–69.4)

10.6 (9.1–13.4)

0.38 (0–1.76)

−1.52 (−2.53–0.45)

−3.75 (−4.02–3.51)

−2.43 (−2.51–2.36)

0.37 (0.35–0.39)

0.568 (0.509–0.636)

  

0.303

DMPC/DMPS/PI(4,5)P 2 3-state + expansion + lag

6.230 (5.000–7.620)

567 (548–581)

8.4 (5.4–12.2)

44.6 (40.1–50.4)

4.6 (2.1–6.4)

20.6 (18.8–33.1)

32.0 (27.1–40.8)

−10.9 (−12.0–10.0)

3.35 (2.81–3.96)

−8.54 (−9.72–7.48)

1.70 (1.55–1.83)

  

15.6 (11.3–19.7)

0.153

DMPC/DMPS/PI(4,5)P2 3-state + expansion + threshold (Fig. 3d)

2.129 (2.041–2.205)

4736 (4448–4926)

382.4 (229.6–500)*

500 (393–500)*

38.9 (23.9–51.7)

13.9 (11.1–17.4

0.55 (0.11–1.00)

−3.06 (−5.07–0.58)

−4.90 (−5.18–4.65)

−3.31 (−3.39–3.16)

0.33 (0.31–0.35)

0.232 (0.226–0.242)

  

0.169

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