Figure 2
From: MicroRNA-33b Inhibits Breast Cancer Metastasis by Targeting HMGA2, SALL4 and Twist1
HMGA2, SALL4 and Twist1 are bona fide downstream targets of miR-33b.
(A) qRT-PCR analysis of the mRNA levels of predicted target genes in BT-549/ctrl and BT-549/miR-33b cells. (B) qRT-PCR analysis of the mRNA levels of predicted target genes in MDA-MB-231/ctrl and MDA-MB-231/miR-33b cells. (C) Dual luciferase reporter assay analysis of the effect of miR-33b expression on the activity of the 3′UTR of target genes in 293T cells. (D) Dual luciferase reporter assay analysis of the effect of miR-33b expression on the activity of the 3′UTR of target genes in MDA-MB-231 cells. (E) Dual luciferase reporter assay analysis of the effect of miR-33b expression on the activity of wild-type and mutant 3′UTRs of HMGA2, SALL4 and Twist1. Data represent the mean ± s.d. *: P <0.05, **: P <0.01, ***: P <0.001, N.S.: no significance.
