Figure 7

miR-33b inhibits tumor growth and lung metastasis of breast cancer cells in vivo.

(A) Bioluminescence images of mice showing primary tumors derived from orthotopic injections into the mammary fat pad of 4T1 control cells (ctrl) or 4T1/mmu-miR-33 cells at day 21 (left). Images of tumors from all mice in each group (middle). Quantitation of tumors from mice in each group (right) (n = 4). (B) Bioluminescence images of mice showing lung metastases at day 35 derived from orthotopic injections of 4T1 control cells and mmu-miR-33-expressing cells. In this case, primary tumors were resected at day 21 (top-left). Quantitation of lung metastases as assessed by Bioluminescence measurements (top-right) (n = 4). Arrows indicate metastatic foci (bottom). (C) Bioluminescence images of animals showing lung metastases at day 14 derived from the tail vein injection of 4T1 control cells and mmu-miR-33-expressing cells (top-left). Quantitation of lung metastases as assessed by bioluminescence measurements (top-right) (n = 4). Arrows indicate metastatic foci (bottom). (D) Bioluminescence images of mice showing primary tumors derived from orthotopic injections into the mammary fat pad of MDA-MB-231 control cells (ctrl) and MDA-MB-231/miR-33b cells at day 28 (left). Images of tumors from all mice in each group (middle). Quantitation of tumors from all mice in each group (right) (n = 5). (E) Bioluminescence images of mice showing lung metastases at day 42 derived from orthotopic injections of MDA-MB-231 control cells and miR-33b-expressing cells. In this case, primary tumors were resected at day 28 (top-left). Quantitation of lung metastases as assessed by bioluminescence measurements at day 42 (top-right) (n = 5). Arrows indicate metastatic foci (bottom). (F) Bioluminescence images of mice showing lung metastases at day 42 derived from the tail vein injection of MDA-MB-231 control cells and miR-33b-expressing cells (top-left). Quantitation of lung metastases as assessed by bioluminescence measurements (top-right) (n = 5). Arrows indicate metastatic foci (bottom). The color scale bar depicts the photon flux (photons per second) emitted from these mice. Scale bars, 100 μm. Data represent the mean ± s.d. *: P <0.05, **: P <0.01, ***: P <0.001.