Table 1 The mRNA expression of Per2, p53, Cyclin D1, CDK1 and Cyclin B1 in groups I, II and III at 6 different time points over 24 h (means ± SD, n = 5).

From: Circadian variations of clock gene Per2 and cell cycle genes in different stages of carcinogenesis in golden hamster buccal mucosa

Gene

Tissue

4HALO

8HALO

12HALO

16HALO

20HALO

24HALO

P -value

Per2

I

0.86 ± 0.06

0.77 ± 0.10

1.89 ± 0.14

2.80 ± 0.11

1.18 ± 0.09

1.54 ± 0.19

0.000

 

II

0.73 ± 0.18

1.33 ± 0.10

1.33 ± 0.19

1.46 ± 0.24

1.12 ± 0.26

0.29 ± 0.18

0.000

 

III

0.35 ± 0.14

1.02 ± 0.13

1.13 ± 0.24

1.47 ± 0.24

1.49 ± 0.25

0.35 ± 0.07

0.000

p53

I

1.09 ± 0.26

1.86 ± 0.27

2.64 ± 0.21

3.82 ± 0.61

4.44 ± 1.40

6.20 ± 1.59

0.000

 

II

2.64 ± 0.63

2.06 ± 0.33

1.22 ± 0.28

2.15 ± 0.20

2.63 ± 0.62

2.94 ± 1.45

0.001

 

III

1.37 ± 0.14

0.84 ± 0.03

0.71 ± 0.10

1.63 ± 0.17

1.82 ± 0.69

1.40 ± 0.34

0.003

Cyclin D1

I

0.86 ± 0.12

0.90 ± 0.12

1.28 ± 0.37

2.51 ± 0.03

1.80 ± 1.40

1.17 ± 0.40

0.000

 

II

2.40 ± 0.14

1.44 ± 0.29

1.36 ± 0.15

1.76 ± 0.16

2.14 ± 0.19

1.67 ± 0.09

0.000

 

III

2.38 ± 0.11

2.20 ± 0.08

2.90 ± 0.16

3.19 ± 0.10

2.64 ± 0.26

2.83 ± 0.24

0.000

CDK1

I

5.91 ± 0.95

5.10 ± 0.45

1.70 ± 0.51

2.40 ± 0.44

1.21 ± 0.33

1.69 ± 0.32

0.000

 

II

5.39 ± 0.55

6.55 ± 0.36

1.33 ± 0.11

2.08 ± 0.54

1.68 ± 0.28

10.0 ± 0.82

0.000

 

III

2.92 ± 0.49

1.68 ± 0.18

3.74 ± 0.36

6.69 ± 0.48

6.36 ± 0.61

7.24 ± 0.87

0.011

Cyclin B1

I

1.16 ± 0.29

1.84 ± 0.12

0.39 ± 0.14

0.61 ± 0.23

1.06 ± 0.10

3.00 ± 0.37

0.000

 

II

1.68 ± 0.27

1.92 ± 0.72

1.51 ± 0.72w

2.00 ± 0.25

1.70 ± 0.25

1.94 ± 0.75

0.53

 

III

2.16 ± 0.64

1.79 ± 0.15

2.14 ± 0.16

2.48 ± 0.78

4.21 ± 1.00

2.55 ± 0.79

0.011

  1. A value of P < 0.05 represents a significant difference ( I: normal buccal mucosa, II: precancerous lesion and III: carcinoma; HALO: hour after light onset; SD: standard deviation; n: numbers).
Back to article page