Figure 5
From: Derivation and characterization of human embryonic stem cells on human amnion epithelial cells
Transmission electron micrographs of GFY-1 on hAECs.
(A) hESCs on hAECs. The surface of the hAECs contained non-intestinal-type microvilli (arrow). The hAECs exhibited a protein-synthesizing phenotype, based on their large cytoplasm. Cell pseudopodia (arrow head) were observed between hAECs and hESCs. (B) hESC colony. In general, hESCs have large nuclei with reticulated nucleoli and scanty cytoplasm. Most of our hESCs exhibited scanty cytoplasm with large nuclei. (C,D) Desmosomes (marked as “D” in images) are specialized cell contacts and dense cell junctions that were present in our hESC colonies. (E) A group of mitochondria was evident in this hESC colony. (F,G) These cells were generally similar to phagocytic cells with lysosomes, Golgi apparatus and rough endoplasmic reticulum (ER). (H) An apoptotic cell was observed in a hESC colony when the number of passages reached 35. Bars: A, H = 2 μm; B = 5 μm; C = 1μm; D, E, G = 0.5 μm.
