Figure 5 | Scientific Reports

Figure 5

From: Discovery and characterization of novel small-molecule inhibitors targeting nicotinamide phosphoribosyltransferase

Figure 5

Monitoring target binding of MS0 analogues in cell lysate and in intact cell.

(A) Representative Western blot of cellular thermal shift assay (CETSA) in cell lysate for NAMPT target with MS1 (at 100 μM). (B) CESTA melt curves in cell lysate for NAMPT target with MS0, MS1 and MS34 (all at 100 μM). (C) Representative Western blot of CETSA in intact cell for NAMPT target with MS1 (at 10 μM). (D) CESTA melt curves in intact cell for NAMPT target with MS0, MS1 and MS34 (all at 10 μM).

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