Figure 8
The PP2A activator devoid of sphingosine 1- phosphate agonism - AAL(s) -overcomes OA-mediated inhibition of basal PP2A phosphatase activity and significantly represses IL-6 and IL-8 mRNA expression and cytokine secretion.
A549 cells were treated for 6 h with 2.5 μM AAL(s) prior to 45 min with 1 μM OA, compared to vehicle. Cells were washed and then (A) PP2A activity measured at 1 h, (B, C) IL-6 and IL-8 mRNA expression measured at 1 h (results expressed as fold increase compared to vehicle-treated cells) and (D, E) IL-6 and IL-8 protein secretion measured at 24 h. Statistical analysis was performed using one-way ANOVA then Bonferroni’s post-test (where * denotes a significant effect of OA or § AAL(s) (P < 0.05)). Data are mean + SEM values from n = 3 independent experiments.
