Figure 3
From: Larynx carcinoma regulates tumor-associated macrophages through PLGF signaling
PLGF polarized macrophages to TAM to activate MMP9 and TGFβ1. (a) RT-qPCR for TGFβ1 and MMP9 in isolated M1MΦ and M2MΦ from the co-culture system. 2 × 104 cells were used for RT-qPCR. (b) ELISA for TGFβ1 and MMP9 in the conditioned media from the co-culture system. (c) Western blot for SMAD3 and phosphorylated SMAD3 (pSMAD3). *: p < 0.05. NS: non-significant. N = 5. Statistic method: one-way ANOVA with a Bonferoni Correction.
