Figure 2
From: Identification of enzymes involved in SUMOylation in Trypanosoma brucei
SDS-PAGE (12%) (Coomassie blue stained) and western blotting analysis of GST pull down samples
a) GST-TbSUMO pull down TbUbc9-His. SDS-PAGE analysis (Coomassie blue stained) of GST-TbSUMO pull down samples (upper). Due to the similar sizes of TbUbc9 and GST, western blotting was performed using anti-His antibody (Lower). GST protein was marked with asterisk (*). (b) Western blotting analysis of GST-TbAos1 pull down samples. GST-TbAos1/TbUba2-His (upper), GST-TbAos1/TbUbc9-His (middle) and GST-TbAos1/TbSUMO-His (lower). GST-apo pull down was used as control.
