Table 1 Mapping of the optimal binding region of the mAb 6D8 on the MSP211-23 sequence using a panel of overlapping synthetic peptides.

From: Structural basis for epitope masking and strain specificity of a conserved epitope in an intrinsically disordered malaria vaccine candidate

Peptide

Sequence∆

Kd (nM)ø

MSP211-23*

FINNAYNMSIRRS

6±3

MSP211-18

FINNAYNM

>1000

MSP212-19

INNAYNMS

>1000

MSP213-20

NNAYNMSI

>1000

MSP214-21

NAYNMSIR

>1000

MSP215-22

AYNMSIRR

87±21

MSP216-23

YNMSIRRS

>1000

MSP215-23

AYNMSIRRS

34±12

MSP214-22

NAYNMSIRR

6±2

MSP213-22

NNAYNMSIRR

14±5

MSP214-23

NAYNMSIRRS

16±7

  1. øKd determined by SPR ± standard deviation of three replicate experiments.
  2. ∆Synthetic peptides were N-terminally acetylated and C-terminally amidated;
  3. *Broad epitope mapped previously22
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