Figure 1

Culture of human minor salivary gland mesenchymal stem cells and expression of stem cell markers in vitro and in vivo.
(a) One of the minor salivary glands dissected during surgery. (b) Explant primary culture for hMSGMSCs at day 4. (c) Expanded hMSGMSCs of passage 3. (d) Cell growth curve for passage 4 hMSGMSCs. (e) Immunostaining of ex vivo expanded hMSGMSCs. (f) Immunostaining of human minor salivary gland tissues for stem cell markers. Positively expressed stem cell markers in hMSGMSCs localize in the basal layer of lower secretory ducts (white arrows).