Figure 3 | Scientific Reports

Figure 3

From: Characterization of a Self-renewing and Multi-potent Cell Population Isolated from Human Minor Salivary Glands

Figure 3

Hepatocyte induction in vitro and in vivo.

Three-stage induction protocol for hepatocyte differentiation (a). Pictures taken on day 1, day 7 and day 14 from left to right(b). Quantitative real-time PCR analysis of relative mRNA expression of endoderm marker genes for different development process on day 7 and day 14 (c). Immunostaining for hepatocyte expressing protein AFP (d) and KRT 18 (e). Indocyanine green (ICG) assay on day 14 of hepatocyte induction (f). Cell image under fluorescence microscope 12 hours post CM-Dil labeling (g). AFP immunostaining 2 weeks after hMSGMSCs transplantation, green fluorescence signal for AFP and red for CM-Dil labeling(h). *P < 0.05, **P < 0.01, ***P < 0.001, **** P < 0.0001, n ≥ 3. Negative control images were show in the white rectangles in d, e and f.

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