Figure 2 | Scientific Reports

Figure 2

From: Combined treatment with a pH-sensitive fusogenic peptide and cationic lipids achieves enhanced cytosolic delivery of exosomes

Figure 2

Increased cellular uptake of exosomes by treatment with cationic lipids.

(a) Confocal microscopic observation of HeLa cells treated with CD63-GFP-exosomes (20 μg/ml) in the presence or absence of Lipofectamine LTX (2 or 4% (v/v)) for 24 h at 37 °C (blue: Hoechst33342, green: CD63-GFP-exosomes). Scale bar: 20 μm. (b) TEM observation of CD63-GFP-exosomes (20 μg/ml) mixed with Lipofectamine LTX (2% (v/v)). Scale bar: 100 nm. (c) Relative cellular uptake of CD63-GFP-exosomes (20 μg/ml) in the presence or absence of Lipofectamine LTX (0.5–4% (v/v)) analysed using a flow cytometer under the same experimental conditions as (a). (d) Cytotoxicity in the combined treatment of exosomes (20 μg/ml) and Lipofectamine LTX (0.5–8% (v/v)) for 24 h at 37 °C of HeLa cells analysed by the WST-1 assay. The data are the averages (±SD) of three (c) and four (d) experiments.

Back to article page