Figure 6
DEAB treatment and aldh1a2 knockout delayed meiotic initiation.
A-R, histological observations of the DEAB-treated and the aldh1a2 knockout fish on meiotic initiation in the XX and XY tilapia. DEAB-treated and aldh1a2 knockout fish resulted in delayed germ cells meiotic initiation in the ovaries to 60 dah and in the testes to 120 dah. OG, oogonia; OC, oocytes; SG, spermatogonia; SC, spermatocytes; ST, spermatids. Scale bar: 10 μm. S-V, the expression of cyp26a1 and meiosis marker sycp3 in the control, the DEAB-treated and the aldh1a2 knockout fish ovaries (30, 60 and 75 dah) and testes (90, 120 and 150 dah) by real-time PCR. Data were expressed as mean ±SD (n = 5). Different letters indicate statistical differences at P < 0.05 as determined by one-way ANOVA followed with a post-hoc test. KO, knockout. Dah, days after hatching.
