Figure 2
Cumulative silencing time distribution of colorectal cancer- and type 2 diabetes mellitus-related proteins, as well as proteins, which are not related to these diseases.
The diagram shows the cumulative distribution of the normalized number of proteins with given silencing times, which are related to the disease (red line), as well as those, which are not related to the disease (green dotted line); for colorectal cancer (Panel A) and type 2 diabetes (Panel B). The number of proteins was normalized by dividing the number of proteins in each silencing time range by the total number of proteins allowing a better comparison. The total number of colorectal cancer-related proteins and type 2 diabetes-related proteins in the human interactome was 18 and 14, respectively. The human interactome containing 12,439 proteins and 174,666 edges was built from the STRING database46. Colorectal cancer- and type 2 diabetes-related proteins were obtained from the Cancer Gene Census database48 and from the article of Parchwani et al.49, respectively. Silencing times were calculated separately for every protein with the Turbine program35 as described in the Methods section using a starting energy of 1,000 and a dissipation value of 5 units. Statistical analysis was performed using the Mann-Whitney (Wilcoxon rank sum) test function of the R package56. There was a statistically significant difference between the silencing times of disease-related and non-related proteins in case of colorectal cancer (p = 2.329e-9) and but there was none in case of type 2 diabetes (p = 0.8343).
