Figure 2

FACS analysis.

Sera from different groups were incubated with transfected HEK293T cells and were assayed by flow cytometry. Cells gated in the upper right (UR) portion of the scatter plot represent double-stained cells, which were considered to be AChR-antibody positive. The cut-off was defined as the mean +3 SDs (=0.98%) of results from 12 healthy controls binding to clustered AChR. (a) Demonstration of the gating. (b) Scatter plots from FACS analysis of the negative control (healthy subject). (c) Scatter plots of a sample from an MG patient, whose serum was anti-AChR positive based on ELISA (AChR-MG), serving as the positive control. (d) A serum sample of an SNMG patient. (e) Summary of FACS analysis results of the three groups. n = 12 in healthy subjects (negative controls), n = 24 in SNMG patients and n = 6 in AChR-MG patients (positive controls).