Figure 1 | Scientific Reports

Figure 1

From: Effect of targeted estrogen delivery using glucagon-like peptide-1 on insulin secretion, insulin sensitivity and glucose homeostasis

Figure 1

GLP-1 efficiently targets E2 to β-cells.

(A) Relative luciferase activity normalized to protein levels in Min6 cells transfected with an ERE-Luc expression plasmid and treated with E2 (10 nM), aGLP1 (10 nM) or aGLP1-E2 (10nM). N = 6 from 2 independent experiments. (B) LRH-1 mRNA expression normalized to β-actin in Min6 cells treated with E2 (10 nM), aGLP1 (10 nM), or aGLP1-E2 (10 nM). N = 3 from 1 experiment. (C) Relative luciferase activity normalized to protein levels in MCF7 human breast cancer cells transfected with an ERE-Luc expression plasmid and treated with E2 (10 nM), aGLP1 (10 nM), or aGLP1-E2 (10 nM). N = 9 from 3 independent experiments (D) Uterus weight in female sham operated and ovariectomized (OVX) mice following two days of treatment with E2 (4 μg/mouse), aGLP1 (120 μg/kg) or aGLP1-E2 (120 μg/kg). (E) ERα mRNA expression normalized to β-actin in uterus from mice in part D. N = 5-12 mice per group. Results represent the mean ± SEM. *≤0.05, **≤0.01, ***≤0.001 vs. Vehicle when not indicated.

Back to article page