Figure 1
Experimental scheme detailing administration of SL327.
(a) At postnatal day 6 (P6), mice were subjected to intraperitoneal (IP) injection with the MEK inhibitor, SL327 (50 mg/kg) or the same amount of vehicle (DMSO). At the end of 6-h period after administration, some groups of mice were sacrificed and assayed for apoptosis or arterial blood gas. Other groups were weaned and allowed to further mature. Then, they were either used for electrophysiological studies at 4 weeks of age or behavioral studies at 9-11 weeks of age. (b) In another experiment, mice were injected with SL327 (50 mg/kg) at P14. Then, some groups were assayed for apoptosis 6 h later similarly to mice administered SL327 at P6. Other group was weaned and allowed to further mature and them, used for behavioral studies at 9-11 weeks of age.
