Table 1 Photophysical parameters of FPs that affect the FRET efficiency and photostability.
From: Optimizing fluorescent protein trios for 3-Way FRET imaging of protein interactions in living cells
Ex λ(nm) | Em λ(nm) | ε(mM−1cm−1) | Q | Photostability (s) | Ref | |
|---|---|---|---|---|---|---|
mCerulean | 433 | 475 | 43 | 0.62 | 36 | |
mTurqoise2 | 434 | 474 | 30 | 0.93 | 90 | |
CyPet | 435 | 477 | 35 | 0.51 | 59 | |
mTFP1 | 462 | 488 | 64 | 0.85 | 110 | |
mCitrine | 516 | 530 | 77 | 0.76 | 49 | |
YPet | 517 | 530 | 104 | 0.77 | 49 | |
TagRFP | 555 | 584 | 100 | 0.48 | 37 | |
TagRFP-t | 555 | 584 | 81 | 0.41 | 337 | |
mRuby2 | 559 | 600 | 113 | 0.38 | 123 | |
mCherry | 587 | 610 | 72 | 0.22 | 96 |
