Figure 2
From: The Galvanotactic Migration of Keratinocytes is Enhanced by Hypoxic Preconditioning
The galvanotactic migration of keratinocytes is promoted by hypoxic preconditioning.
Keratinocytes were first pre-cultured in 1%, 2% or 5% O2 for 6 hours and then stimulated by an EF of 50 mV/mm for another 3 hours under normoxic conditions. (A–D) The migration trajectories of keratinocytes guided by EFs with hypoxic preconditioning (B–D) or not (A). (E–H) Analysis of the directedness (E), trajectory speed (F), displacement speed (G) and X-axis speed (H) of keratinocyte migration. (I–J) Time course of the directedness (I) and X-axis speed (J) of keratinocyte migration guided by EF under hypoxic preconditioning (2% O2, 6 hours) or normoxic culture conditions. (K) Illustration of the methods used for the quantification of cell migration. Also see the Materials and Methods section, “Quantitative analysis of cell migration” for details. The data are from at least 100 cells in 3 independent experiments and are shown as the mean ± SEM. *, p < 0.05; #, p < 0.01 compared with the cells without hypoxia pretreatment.
