Figure 6
From: The Galvanotactic Migration of Keratinocytes is Enhanced by Hypoxic Preconditioning
ROS are linked to hypoxic preconditioning with enhanced keratinocyte galvanotaxis.
(A–D) Representative images showing the staining of intracellular superoxide by HE (red fluorescence) in keratinocytes in the absence or presence of NAC (2 mM) when preconditioned by hypoxia (2% O2, 6 hours) or not. (E–H) Representative images showing the staining of intracellular hydrogen peroxide by DCFH-DA (green fluorescence) in keratinocytes in the absence or presence of NAC (2 mM) when preconditioned by hypoxia (2% O2, 6 hours) or not. (I–J) Analysis of the normalized directedness (I) or trajectory speed (J) of keratinocyte migration guided by EF (50 mV/mm) in the absence or presence of NAC when the cells were preconditioned by hypoxia (2% O2, 6 hours) or not (normoxia). The data are from at least 100 cells in 3 independent experiments and are shown as the mean ± SEM. #, p < 0.01 compared with the cells treated with DMSO. Bar = 25 μm.
