Figure 2
Iturin A inhibits proliferation of breast cancer cells.
(A) Chemical structure of Iturin A consists of peptide chain containing seven amino acids linked to a fatty acid component. (B) MDA-MB-231, MCF-7, MDA-MB-468 and T47D breast cancer cells are treated with different concentration of Iturin A for 48 h. Following drug exposure, MTT solution is added and cells are incubated for 4 h. 100 μl DMSO was added after discarding MTT solution. Dissolved formazan is determined spectrophotometrically to measure the cell viability in treated groups compared to control. (C) Breast cancer cells are treated with IC50 dose of Iturin A and normal cells are treated with higher dose of IC50 dose of breast cancer cells. Representative histograms of MDA-MB-231, MCF-7, HMEC and HaCaT cells and their cell-cycle distribution after 12, 24 and 48 h of treatment are shown. (D) Graphical representation of Sub G1 populations (% of Apoptotic cells) in different cell lines from flow cytometric analysis. (E) Fluorescence microscopic analysis of treated MDA-MB-231 and MCF-7 cancer cells as well as HMEC and HaCaT normal cells (20 × magnification). Values are mean ± S.D of three independent experiments. Significance level is represented by *P<0.05, **P<0.01, ***P<0.001.
