Figure 5 | Scientific Reports

Figure 5

From: Marine lipopeptide Iturin A inhibits Akt mediated GSK3β and FoxO3a signaling and triggers apoptosis in breast cancer

Figure 5

Transfection study.

(A) MDA-MB-231 and MCF-7 cells are transfected with pcDNA, pcDNA-Akt and siRNA-Akt following Iturin A treatment. Expression levels of P-Akt (Ser 473 and Thr 308) and P-MAPK are evaluated by immunoblotting. β-Actin is used as loading control. Blots were cut and cropped according to the molecular weight of the proteins. (B) and (C) Densitometric plot of P-Akt (Ser 473 and Thr 308) and P-MAPK levels in both cell lines. Each bar represents mean ± S.D from three different experiments.

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